Plant anatomy and biology lab journal of Cells (crystal, anthocyanin, plasmolysis, isoloasi cell protoplasts)
I. pratikum Date: March 10, 2016
II. Pratikum Title: Cells (crystal, anthocyanin, plasmolysis, isoloasi cell protoplasts)
III. Objective pratikum:
1. To
observe crystal sand
2. To observe the crystal drusen (star-shaped)
3. To observe the needle crystals (rafida)
4. To observe sistolit
5. To observe the anthocyanin
6. To observe events plasmolysis
2. To observe the crystal drusen (star-shaped)
3. To observe the needle crystals (rafida)
4. To observe sistolit
5. To observe the anthocyanin
6. To observe events plasmolysis
IV. Basic theory :
Plasmolysis
is an event occurrence mempran shrunken cytoplasm and the loss of plasma from
the walls of plant cells if the cell is inserted in a hypertonic solution. Plasmolysis
is also a process that obviously could indicate that the cell as the smallest
unit of life (Haspari, 2008: 67).
Anthocyanins are pigments carrier purplish red color in fruits, flavonoids that can protect cells from ultravioled. Having a good antioxidant effect because it can inhibit free radicals (Novianti, 2005: 178).
Protoplasm is a plant cell which has been separated from the cell wall or plant cells without cell walls wrapped, prtoplasma very trivial in the field of genetic engineering (Sukamadjaja, 2006: 245).
Anthocyanins are pigments carrier purplish red color in fruits, flavonoids that can protect cells from ultravioled. Having a good antioxidant effect because it can inhibit free radicals (Novianti, 2005: 178).
Protoplasm is a plant cell which has been separated from the cell wall or plant cells without cell walls wrapped, prtoplasma very trivial in the field of genetic engineering (Sukamadjaja, 2006: 245).
V.
Tools
and Materials:
A. Tool
1. Microscope
2. Glass objects
3. Razor
4. Pipette drops
5. Glass objects
6. Glass cover
7. washcloth or tissue
B. Materials
1. A cross section stem eggplant (Solanum tuberosum) and petiole Begonia sp
2. A cross section Carica papaya papaya leaf stalks in water
3. The cross section leaves of Mirabilis jalapa
4. A cross section of leaves Ficus Elastica in water
5. The cross-section of the underside of leaves Rhoe discolor
6. The cross-section of the underside of leaves Rhoe discolor in sucrose 5%
7. Leaves chili
8. Leaves of green beans
VI. Ways of working
1. Crystals
a. Transverse incision made preparations
b. Placed on the glass of the object
c. Drops of water and cover with glass cover
d. And then observed with a microscope
2. Anthocyanins
a. An incision is made below the surface of the leaf 3 adam hawa
b. The third glass is placed on different objects, glass objects 1 by droplets of distilled water, the slide 2 by droplets of aqueous KOH 1% and the latter by HCL 1% silenced one minute.
c. Observed under the microscope.
3. plasmolysis
a. 2 pieces incision is made under the leaves Rhoe permukaana discolor
b. Placed on the slide
c. Glass objects first drops of water, two drops of 5% sucrose covered with cover glass and observed under a microscope.
4. Isolation cells and protoplasm
a. Washed seeds chili and green beans with distilled water and 20% Clorox
b. Chili seeds planted in a glass jar containing rice paper and then allowed to grow approximately 3-5 days
c. Settling leaves, chopped and placed in petridish by distilled water and observed under a microscope
a. Transverse incision made preparations
b. Placed on the glass of the object
c. Drops of water and cover with glass cover
d. And then observed with a microscope
2. Anthocyanins
a. An incision is made below the surface of the leaf 3 adam hawa
b. The third glass is placed on different objects, glass objects 1 by droplets of distilled water, the slide 2 by droplets of aqueous KOH 1% and the latter by HCL 1% silenced one minute.
c. Observed under the microscope.
3. plasmolysis
a. 2 pieces incision is made under the leaves Rhoe permukaana discolor
b. Placed on the slide
c. Glass objects first drops of water, two drops of 5% sucrose covered with cover glass and observed under a microscope.
4. Isolation cells and protoplasm
a. Washed seeds chili and green beans with distilled water and 20% Clorox
b. Chili seeds planted in a glass jar containing rice paper and then allowed to grow approximately 3-5 days
c. Settling leaves, chopped and placed in petridish by distilled water and observed under a microscope
VII. Observation result
VIII.
Discussion
The cell is the smallest structural integrity is physiological in a living
organism. Disususn by cell protoplasm and the cell wall. Ie the whole of cell
protoplasm consisting of the cytoplasm, the protoplasm are organle-cell
organelles such as mitochondria, plastids and others. In non protoplasts
contained vacuoles and ergastik substances.
Practicum this time we observed the crystals and antosiani. Crystals that we
observed there are several kinds of crystal sand, crystal drusen, crystalline
needles, anthocyanin, sistolit and events plasmolysis. Crystals are usually
composed of calcium oxalate formed due to excess cambium inside the cell which
is then attached to the products of metabolism such as oxalic acid which in
high levels and harmful to the cell.
Crystals are generally abundant in plant
cells and dissolved in the liquid cell. These pigments can cause pink, red
purple of organs in plants.
On the stem Solanum ningrum, we observed and found crystal sand and spread in
the cell. At stalk Carica papaya, we observed drusen crystal contained in a
star-shaped cells. On leaves Ficus Elastica we observed sistolit exist in the
leaf tissue and there are clumps of crystals near the epidermal tissue. On the
underside of leaves Rhoe discolor, we observed anthocyanin in purple when coupled
ordinary water, but then we mn = encoba added with a solution of HCl 1% and
then the purple color begins to fade. And last we add KOH 1% then the purple color
disappears.
Practicum this time we observed the crystals and antosiani. Crystals that we observed there are several kinds of crystal sand, crystal drusen, crystalline needles, anthocyanin, sistolit and events plasmolysis. Crystals are usually composed of calcium oxalate formed due to excess cambium inside the cell which is then attached to the products of metabolism such as oxalic acid which in high levels and harmful to the cell.
On the stem Solanum ningrum, we observed and found crystal sand and spread in the cell. At stalk Carica papaya, we observed drusen crystal contained in a star-shaped cells. On leaves Ficus Elastica we observed sistolit exist in the leaf tissue and there are clumps of crystals near the epidermal tissue. On the underside of leaves Rhoe discolor, we observed anthocyanin in purple when coupled ordinary water, but then we mn = encoba added with a solution of HCl 1% and then the purple color begins to fade. And last we add KOH 1% then the purple color disappears.
The cell is the smallest structural integrity is physiological in a living organism. Disususn by cell protoplasm and the cell wall. Ie the whole of cell protoplasm consisting of the cytoplasm, the protoplasm are organle-cell organelles such as mitochondria, plastids and others. In non protoplasts contained vacuoles and ergastik substances.
Practicum this time we observed the crystals and antosiani. Crystals that we observed there are several kinds of crystal sand, crystal drusen, crystalline needles, anthocyanin, sistolit and events plasmolysis. Crystals are usually composed of calcium oxalate formed due to excess cambium inside the cell which is then attached to the products of metabolism such as oxalic acid which in high levels and harmful to the cell. Crystals are generally abundant in plant cells and dissolved in the liquid cell. These pigments can cause pink, red purple of organs in plants.
On the stem Solanum ningrum, we observed and found crystal sand and spread in the cell. At stalk Carica papaya, we observed drusen crystal contained in a star-shaped cells. On leaves Ficus Elastica we observed sistolit exist in the leaf tissue and there are clumps of crystals near the epidermal tissue. On the underside of leaves Rhoe discolor, we observed anthocyanin in purple when coupled ordinary water, but then we mn = encoba added with a solution of HCl 1% and then the purple color begins to fade. And last we add KOH 1% then the purple color disappears.
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